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enterococcus faecalis atcc van b v583 e  (ATCC)
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ATCC enterococcus faecalis atcc van b v583 e
Enterococcus Faecalis Atcc Van B V583 E, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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collagenolytic e faecalis strain v583  (ATCC)
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ATCC collagenolytic e faecalis strain v583
A) E. <t>faecalis</t> in the presence of 0 (red), 2mM (orange) and 10mM (blue) TXA and FITC-labeled PLG. Flow cytometry demonstrated that TXA concentration-dependently inhibited binding of PLG to E. faecalis (* p<0.05 compared to 0mM TXA, K-S analysis). B) TXA concentration-dependently and significantly, by over 30% at the maximum dose tested - 10mM, inhibits PLG activation by E. faecalis in the presence of uPA (* p<0.05, ANCOVA). C) TXA concentration-dependently inhibits type IV collagen degradation by E. faecalis in the presence of PLG and uPA (* p<0.05, ANCOVA). 10mM TXA almost completely eliminated bacterial plasmin-dependent collagen degradation D) Treatment with TXA had no impact on 6 hour population growth of E. faecalis. E) Treatment with TXA had no impact on collagen degradation by E. faecalis in the absence of PLG. H) In a model of E. faecalis-induced anastomotic leak, mice treated with TXA enema exhibited improved anastomotic healing scores compared to mice receiving vehicle (water) control (1.5 ± 0.5 vs 2.7 ± 0.6, *p<0.05 Student’s t-test). Each point represents one animal (n=10 per group). In this model, clinically relevant anastomotic leak is defined as AHS 3 or above. Thus, 60% of mice receiving control treatment suffered clinically relevant leak while 0% of TXA-treated mice demonstrated this outcome (p<0.01 Fisher’s exact test). Error bars indicate means ± SEM. All in vitro data are representative of three separate experiments, each run in triplicate.
Collagenolytic E Faecalis Strain V583, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e%2E+faecalis+strain+v583/Enterococcus+faecalis/pmc07875208-123-2-7
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e faecalis genomic dna  (ATCC)
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ATCC e faecalis genomic dna
A) E. <t>faecalis</t> in the presence of 0 (red), 2mM (orange) and 10mM (blue) TXA and FITC-labeled PLG. Flow cytometry demonstrated that TXA concentration-dependently inhibited binding of PLG to E. faecalis (* p<0.05 compared to 0mM TXA, K-S analysis). B) TXA concentration-dependently and significantly, by over 30% at the maximum dose tested - 10mM, inhibits PLG activation by E. faecalis in the presence of uPA (* p<0.05, ANCOVA). C) TXA concentration-dependently inhibits type IV collagen degradation by E. faecalis in the presence of PLG and uPA (* p<0.05, ANCOVA). 10mM TXA almost completely eliminated bacterial plasmin-dependent collagen degradation D) Treatment with TXA had no impact on 6 hour population growth of E. faecalis. E) Treatment with TXA had no impact on collagen degradation by E. faecalis in the absence of PLG. H) In a model of E. faecalis-induced anastomotic leak, mice treated with TXA enema exhibited improved anastomotic healing scores compared to mice receiving vehicle (water) control (1.5 ± 0.5 vs 2.7 ± 0.6, *p<0.05 Student’s t-test). Each point represents one animal (n=10 per group). In this model, clinically relevant anastomotic leak is defined as AHS 3 or above. Thus, 60% of mice receiving control treatment suffered clinically relevant leak while 0% of TXA-treated mice demonstrated this outcome (p<0.01 Fisher’s exact test). Error bars indicate means ± SEM. All in vitro data are representative of three separate experiments, each run in triplicate.
E Faecalis Genomic Dna, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e%2E+faecalis+strain+v583/Genomic+DNA+from+Enterococcus+faecalis+strain+V583/us10774345-1286-6-10
Average 99 stars, based on 1 article reviews
e faecalis genomic dna - by Bioz Stars, 2026-09
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A) E. faecalis in the presence of 0 (red), 2mM (orange) and 10mM (blue) TXA and FITC-labeled PLG. Flow cytometry demonstrated that TXA concentration-dependently inhibited binding of PLG to E. faecalis (* p<0.05 compared to 0mM TXA, K-S analysis). B) TXA concentration-dependently and significantly, by over 30% at the maximum dose tested - 10mM, inhibits PLG activation by E. faecalis in the presence of uPA (* p<0.05, ANCOVA). C) TXA concentration-dependently inhibits type IV collagen degradation by E. faecalis in the presence of PLG and uPA (* p<0.05, ANCOVA). 10mM TXA almost completely eliminated bacterial plasmin-dependent collagen degradation D) Treatment with TXA had no impact on 6 hour population growth of E. faecalis. E) Treatment with TXA had no impact on collagen degradation by E. faecalis in the absence of PLG. H) In a model of E. faecalis-induced anastomotic leak, mice treated with TXA enema exhibited improved anastomotic healing scores compared to mice receiving vehicle (water) control (1.5 ± 0.5 vs 2.7 ± 0.6, *p<0.05 Student’s t-test). Each point represents one animal (n=10 per group). In this model, clinically relevant anastomotic leak is defined as AHS 3 or above. Thus, 60% of mice receiving control treatment suffered clinically relevant leak while 0% of TXA-treated mice demonstrated this outcome (p<0.01 Fisher’s exact test). Error bars indicate means ± SEM. All in vitro data are representative of three separate experiments, each run in triplicate.

Journal: Annals of surgery

Article Title: Prevention of anastomotic leak via local application of tranexamic acid to target bacterial-mediated plasminogen activation: a practical solution to a complex problem.

doi: 10.1097/SLA.0000000000003733

Figure Lengend Snippet: A) E. faecalis in the presence of 0 (red), 2mM (orange) and 10mM (blue) TXA and FITC-labeled PLG. Flow cytometry demonstrated that TXA concentration-dependently inhibited binding of PLG to E. faecalis (* p<0.05 compared to 0mM TXA, K-S analysis). B) TXA concentration-dependently and significantly, by over 30% at the maximum dose tested - 10mM, inhibits PLG activation by E. faecalis in the presence of uPA (* p<0.05, ANCOVA). C) TXA concentration-dependently inhibits type IV collagen degradation by E. faecalis in the presence of PLG and uPA (* p<0.05, ANCOVA). 10mM TXA almost completely eliminated bacterial plasmin-dependent collagen degradation D) Treatment with TXA had no impact on 6 hour population growth of E. faecalis. E) Treatment with TXA had no impact on collagen degradation by E. faecalis in the absence of PLG. H) In a model of E. faecalis-induced anastomotic leak, mice treated with TXA enema exhibited improved anastomotic healing scores compared to mice receiving vehicle (water) control (1.5 ± 0.5 vs 2.7 ± 0.6, *p<0.05 Student’s t-test). Each point represents one animal (n=10 per group). In this model, clinically relevant anastomotic leak is defined as AHS 3 or above. Thus, 60% of mice receiving control treatment suffered clinically relevant leak while 0% of TXA-treated mice demonstrated this outcome (p<0.01 Fisher’s exact test). Error bars indicate means ± SEM. All in vitro data are representative of three separate experiments, each run in triplicate.

Article Snippet: Commercially available collagenolytic E. faecalis strain V583 (ATCC 700802) was utilized in all experiments.

Techniques: Labeling, Flow Cytometry, Concentration Assay, Binding Assay, Activation Assay, Control, In Vitro

Mice underwent a model of E. faecalis-mediated anastomotic leak and treatment with TXA or vehicle (water) enema. Endpoint colony counts of collagenolytic E. faecalis were performed at sacrifice on postoperative days 3 and 8. A) At postoperative day 3, treatment with TXA did not alter the concentration of collagenolytic E. faecalis in anastomotic tissue. B) By postoperative day 8 a significant difference in total CFU/mg of collagenolytic E. faecalis was observed in the TXA-treated group, possibly indicative of decreased tissue penetrance by bacteria or recovery of the normal microbiome in the setting of a healed anastomosis. C) Mice treated with TXA had significantly fewer CFU/mg collagenolytic E. faecalis in anastomotic luminal contents at postoperative day 3. D) Treatment group animals had persistently low CFU/mg, however this failed to reach statistical significance by postoperative day 8. Each data point represents 1 animal. * p<0.05 Student’s t-test. Error bars indicate 95% confidence intervals.

Journal: Annals of surgery

Article Title: Prevention of anastomotic leak via local application of tranexamic acid to target bacterial-mediated plasminogen activation: a practical solution to a complex problem.

doi: 10.1097/SLA.0000000000003733

Figure Lengend Snippet: Mice underwent a model of E. faecalis-mediated anastomotic leak and treatment with TXA or vehicle (water) enema. Endpoint colony counts of collagenolytic E. faecalis were performed at sacrifice on postoperative days 3 and 8. A) At postoperative day 3, treatment with TXA did not alter the concentration of collagenolytic E. faecalis in anastomotic tissue. B) By postoperative day 8 a significant difference in total CFU/mg of collagenolytic E. faecalis was observed in the TXA-treated group, possibly indicative of decreased tissue penetrance by bacteria or recovery of the normal microbiome in the setting of a healed anastomosis. C) Mice treated with TXA had significantly fewer CFU/mg collagenolytic E. faecalis in anastomotic luminal contents at postoperative day 3. D) Treatment group animals had persistently low CFU/mg, however this failed to reach statistical significance by postoperative day 8. Each data point represents 1 animal. * p<0.05 Student’s t-test. Error bars indicate 95% confidence intervals.

Article Snippet: Commercially available collagenolytic E. faecalis strain V583 (ATCC 700802) was utilized in all experiments.

Techniques: Concentration Assay, Bacteria

PLG activating and collagenolytic bacteria are prevalent in human anastomotic tissue. Ten patients had the mucosal surface of resected colon specimens swabbed for microbiological analysis. Of the ten samples, two were culture negative. Of the remaining 8, all samples harbored species with well described PLG activation in their colonic mucosa.

Journal: Annals of surgery

Article Title: Prevention of anastomotic leak via local application of tranexamic acid to target bacterial-mediated plasminogen activation: a practical solution to a complex problem.

doi: 10.1097/SLA.0000000000003733

Figure Lengend Snippet: PLG activating and collagenolytic bacteria are prevalent in human anastomotic tissue. Ten patients had the mucosal surface of resected colon specimens swabbed for microbiological analysis. Of the ten samples, two were culture negative. Of the remaining 8, all samples harbored species with well described PLG activation in their colonic mucosa.

Article Snippet: Commercially available collagenolytic E. faecalis strain V583 (ATCC 700802) was utilized in all experiments.

Techniques: Bacteria, Activation Assay